Type 3 Secretion Systems: Methods & Protocols download

Download Type 3 Secretion Systems: Methods & Protocols
  • Upload date: 9-11-2017, 19:32
  • Category: Biology
Author: Danielle Condry and Matthew L. Nilles  |  Isbn: 9781493966479  |  File size: 6MB  |  Year: 2017  |  Pages: 224  |  Language: English  |  File format: PDF  |  Category: Biology

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive,Type 3 Secretion Systems: Methods and Protocolsis a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.

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